As the amino acid composition determines the properties of every individual peptide, testing of
peptide solubility with a small amount of product is recommended!
Allow the peptide to warm to room temperature (preferably in a desiccator) before reconstituting.
Always use sterile water or buffer (PBS, Tris or phosphate, pH 7) for preparation of solutions.
For peptides containing Cys, Met or Trp, that are rapidly susceptible to oxidation, oxygen-free solvents should be used.
Solubility can also be facilitated by careful warming (< 40C) or sonication. If the pH of the solution has to be increased, only very weak bases should be used in order to prevent immediate inactivation by racemisation or side reactions.
Guideline for solubilizing your peptide:
1.
Peptides that are shorter than 5 residues are generally soluble in aqueous media, except in extreme cases where all the residues are very hydrophobic.
2.Hydrophilic peptides containing >25% charged residues and 75%) proportion of S,T,E,D,K,R,H,N,Q or Y are capable of forming extensive intermolecular hydrogen bonding network and have a tendency to form gels in concentrated aqueous solutions. These peptides may have to be treated similarly to step
